High-Content Screening and Mechanism-Based Evaluation of Estrogenic Botanical Extracts
نویسندگان
چکیده
منابع مشابه
Microscopy-Based High-Content Screening
Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied to study diverse processes, such as protein-localization changes, cancer cell vulnerabilities, a...
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Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clo...
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Visual inspection of cells is a fundamental tool for discovery in biological science. Modern robotic microscopes are able to capture thousands of images from massively parallel experiments such as RNA interference (RNAi) or small-molecule screens. Such screens also benefit from lab automation, making large screens, e.g., genome-scale knockdown experiments, more feasible and common. As such, the...
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ژورنال
عنوان ژورنال: Combinatorial Chemistry & High Throughput Screening
سال: 2008
ISSN: 1386-2073
DOI: 10.2174/138620708784246022